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LPO ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Lactoperoxidase

Gene Aliases

Lactoperoxidase; lactoperoxydase.

Gene ID

280844

Accession Number

NP_776358.1

Reactivity

Bos taurus, Bovine

Target

Lactoperoxidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LPO ELISA Kit (Bovine) (OKEH03829) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LPO has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LPO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LPO captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000ng/mL

Sensitivity

6.6 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

81 kDa

Specificity

Natural and recombinant Bovine Lactoperoxidase

Protein Length

712

NCBI Gene Symbol

LPO

Protein Name

P80025

Gene Name URL

LPO

Nucleotide Accession Number

NM_173933.2

Frequently Asked Questions

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