BDNF ELISA Kit (Pig)
Product Specifications
CAS Number
7732-18-5
Gene Name
Brain derived neurotrophic factor
Gene Aliases
Brain-derived neurotrophic factor.
Gene ID
397495
Accession Number
NP_999424.1
Reactivity
Porcine, Sus scrofa
Target
Brain-derived neurotrophic factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BDNF ELISA Kit (Pig) (OKEH03796) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BDNF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BDNF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.064 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Pig Brain-derived neurotrophic factor
Protein Length
252
NCBI Gene Symbol
BDNF
Protein Name
P14082
Gene Name URL
BDNF
Nucleotide Accession Number
NM_214259.2
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