What is the catalog number for TSHB ELISA Kit (Bovine)?

The catalog number for TSHB ELISA Kit (Bovine) is OKEH03756-96W.

What sizes are available for TSHB ELISA Kit (Bovine)?

TSHB ELISA Kit (Bovine) is available in the following size(s): 96 Well.

Is TSHB ELISA Kit (Bovine) in stock?

TSHB ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

TSHB ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Thyroid stimulating hormone subunit beta

Gene Aliases

Thyroid stimulating hormone beta; thyroid stimulating hormone, beta polypeptide; Thyroid-stimulating hormone subunit beta; thyrotropin beta chain; thyrotropin beta subunit; thyrotropin subunit beta; TSH-B; TSH-beta.

Gene ID

281552

Accession Number

NP_776630.1

Reactivity

Bos taurus, Bovine

Target

Thyrotropin subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TSHB ELISA Kit (Bovine) (OKEH03756) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine TSHB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TSHB and incubated. The found in the sample or standards competes with the biotinylated TSHB for limited binding sites on the immobilized anti-Bovine TSHB antibody. Excess unbound biotinylated TSHB and sample or standard TSHB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TSHB captured in the well and inversely proportional to the amount of TSHB which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol