GCK ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Glucokinase
Gene Aliases
GLK; glucokinase variant 3; GLUKA; hexokinase type IV; hexokinase-4; hexokinase-D; HK IV; HK4; RNGK2.
Gene ID
24385
Accession Number
NP_001257779.1
Reactivity
Rat, Rattus norvegicus
Target
Glucokinase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GCK ELISA Kit (Rat) (OKEH03746) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gck has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gck is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gck captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
55 kDa
Specificity
Natural and recombinant Rat Glucokinase
Protein Length
498
NCBI Gene Symbol
Gck
Protein Name
P17712
Gene Name URL
Gck
Nucleotide Accession Number
NM_001270850.1
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