GIN1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Gypsy retrotransposon integrase 1
Gene Aliases
GIN-1; gypsy retrotransposon integrase-like protein 1; RGD1307449.
Gene ID
316687
Accession Number
NP_001020183.1
Reactivity
Rat, Rattus norvegicus
Target
Gypsy retrotransposon integrase-like protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology GIN1 ELISA Kit (Rat) (OKEH03744) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Gin1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gin1 and incubated. The Gin1 found in the sample or standards competes with the biotinylated Gin1 for limited binding sites on the immobilized anti-human Gin1 antibody. Excess unbound biotinylated Gin1 and sample or standard Gin1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gin1 captured in the well and inversely proportional to the amount of Gin1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/ml
Sensitivity
0.397 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 kDa
Specificity
Natural and recombinant Rat Gypsy retrotransposon integrase-like protein 1
Protein Length
518
NCBI Gene Symbol
Gin1
Protein Name
Q66H30
Gene Name URL
Gin1
Nucleotide Accession Number
NM_001025012.1
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