What is the catalog number for DGKA ELISA Kit (Rat)?

The catalog number for DGKA ELISA Kit (Rat) is OKEH03740-96W.

What sizes are available for DGKA ELISA Kit (Rat)?

DGKA ELISA Kit (Rat) is available in the following size(s): 96 Tests.

Is DGKA ELISA Kit (Rat) in stock?

DGKA ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

DGKA ELISA Kit (Rat)

Selected Variant

OKEH03740-96W

Size: 96 Tests

Product Specifications

CAS Number

7732-18-5

Gene Name

Diacylglycerol kinase, alpha

Gene Aliases

80 kDa diacylglycerol kinase; DAG kinase alpha; Dagk1; DGK-alpha; diacylglycerol kinase alpha; diglyceride kinase alpha.

Gene ID

140866

Accession Number

NP_542965.1

Reactivity

Rat, Rattus norvegicus

Target

Diacylglycerol kinase alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DGKA ELISA Kit (Rat) (OKEH03740) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dgka has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Dgka is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dgka captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Sensitivity

0.317 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

82 kDa

Specificity

Natural and recombinant Rat Diacylglycerol kinase alpha

Protein Length

727

NCBI Gene Symbol

Dgka

Protein Name

P51556

Gene Name URL

Dgka

Nucleotide Accession Number

NM_080787.1

Price

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