COL4A1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Collagen type IV alpha 1 chain
Gene Aliases
Alpha 1 type IV collagen; collagen alpha-1 (IV) chain; collagen, type IV, alpha 1; procollagen, type IV, alpha 1.
Gene ID
290905
Accession Number
NP_001128481.1
Reactivity
Rat, Rattus norvegicus
Target
Collagen type IV alpha 1 chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology COL4A1 ELISA Kit (Rat) (OKEH03698) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL4A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for COL4A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COL4A1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
0.83 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
161 kDa
Specificity
Natural and recombinant Rat Collagen alpha-1 (IV) chain
Protein Length
1669
NCBI Gene Symbol
Col4a1
Protein Name
F1MA59
Gene Name URL
Col4a1
Nucleotide Accession Number
NM_001135009.1
Frequently Asked Questions
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