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COL4A1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Collagen type IV alpha 1 chain

Gene Aliases

Alpha 1 type IV collagen; collagen alpha-1 (IV) chain; collagen, type IV, alpha 1; procollagen, type IV, alpha 1.

Gene ID

290905

Accession Number

NP_001128481.1

Reactivity

Rat, Rattus norvegicus

Target

Collagen type IV alpha 1 chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology COL4A1 ELISA Kit (Rat) (OKEH03698) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL4A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for COL4A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COL4A1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/mL

Sensitivity

0.83 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

161 kDa

Specificity

Natural and recombinant Rat Collagen alpha-1 (IV) chain

Protein Length

1669

NCBI Gene Symbol

Col4a1

Protein Name

F1MA59

Gene Name URL

Col4a1

Nucleotide Accession Number

NM_001135009.1

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