EEA1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Early endosome antigen 1
Gene Aliases
A430109M19Rik; B230358H09Rik; early endosome antigen 1; ZFYVE; ZFYVE2.
Gene ID
216238
Accession Number
NP_001001932.1
Reactivity
Mouse, Mus musculus
Target
Early endosome antigen 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology EEA1 ELISA Kit (Mouse) (OKEH03694) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Eea1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Eea1 and incubated. The Eea1 found in the sample or standards competes with the biotinylated Eea1 for limited binding sites on the immobilized anti-human Eea1 antibody. Excess unbound biotinylated Eea1 and sample or standard Eea1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Eea1 captured in the well and inversely proportional to the amount of Eea1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.17 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
161 kDa
Specificity
Natural and recombinant Mouse Early endosome antigen 1
Protein Length
1411
NCBI Gene Symbol
Eea1
Protein Name
Q8BL66
Gene Name URL
Eea1
Nucleotide Accession Number
NM_001001932.3
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