NSMAF ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Neutral sphingomyelinase (N-SMase) activation associated factor
Gene Aliases
AA959567; C630007J05; F; factor associated with neutral sphingomyelinase activation; factor associated with N-SMase activation; Fan; protein FAN.
Gene ID
18201
Accession Number
NP_035075.2
Reactivity
Mouse, Mus musculus
Target
Protein FAN
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NSMAF ELISA Kit (Mouse) (OKEH03688) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nsmaf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Nsmaf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nsmaf captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
Sensitivity
39.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
105 kDa
Specificity
Natural and recombinant Mouse Protein FAN
Protein Length
920
NCBI Gene Symbol
Nsmaf
Protein Name
O35242
Gene Name URL
Nsmaf
Nucleotide Accession Number
NM_010945.2
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