EIF4EBP1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Eukaryotic translation initiation factor 4E binding protein 1
Gene Aliases
4e-bp;4e-bp1; AA959816; eIF4E-binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; PH; PHAS-I; phosphorylated heat- and acid-stable protein regulated by insulin 1.
Gene ID
13685
Accession Number
NP_031944.3
Reactivity
Mouse, Mus musculus
Target
Eukaryotic translation initiation factor 4E-binding protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EIF4EBP1 ELISA Kit (Mouse) (OKEH03677) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eif4ebp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Eif4ebp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eif4ebp1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Mouse Eukaryotic translation initiation factor 4E-binding protein 1
Protein Length
117
NCBI Gene Symbol
Eif4ebp1
Protein Name
Q60876
Gene Name URL
Eif4ebp1
Nucleotide Accession Number
NM_007918.3
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