What is the catalog number for FGF22 ELISA Kit (Mouse)?

The catalog number for FGF22 ELISA Kit (Mouse) is OKEH03676-96W.

What sizes are available for FGF22 ELISA Kit (Mouse)?

FGF22 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is FGF22 ELISA Kit (Mouse) in stock?

FGF22 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

FGF22 ELISA Kit (Mouse)

Selected Variant

OKEH03676-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Fibroblast growth factor 22

Gene Aliases

2210414E06Rik; FGF-22; Fgf5d; fibroblast growth factor 22; fibroblast growth factor 5d.

Gene ID

67112

Accession Number

NP_075793.1

Reactivity

Mouse, Mus musculus

Target

Fibroblast growth factor 22

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FGF22 ELISA Kit (Mouse) (OKEH03676) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgf22 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fgf22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgf22 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

19 kDa

Specificity

Natural and recombinant Mouse Fibroblast growth factor 22

Protein Length

162

NCBI Gene Symbol

Fgf22

Protein Name

Q9ESS2

Gene Name URL

Fgf22

Nucleotide Accession Number

NM_023304.1

Price

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