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ICOSLG ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Icos ligand

Gene Aliases

AU044799; B7 homolog 2; B7h; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP; B7RP-1; BG071784; GI50; GL5; GL50; GL50-; GL50-B; IC; ICOS ligand; ICOS-L; Icoslg; L; LICOS; Ly115l; mKIAA0653.

Gene ID

50723

Accession Number

NP_056605.1

Reactivity

Mouse, Mus musculus

Target

ICOS ligand

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ICOSLG ELISA Kit (Mouse) (OKEH03668) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Icoslg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Icoslg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Icoslg captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

0.095 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

36 kDa

Specificity

Natural and recombinant Mouse ICOS ligand

Protein Length

322

NCBI Gene Symbol

Icosl

Protein Name

Q9JHJ8

Gene Name URL

Icosl

Nucleotide Accession Number

NM_015790.3

Frequently Asked Questions

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