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HLA-DRB1-9 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Major histocompatibility complex, class II, DR beta 1

Gene Aliases

DRB1; HLA class II histocompatibility antigen, DR-1 beta chain; HLA-DR1B; HLA-DRB; human leucocyte antigen DRB1; lymphocyte antigen DRB1; major histocompatibility complex, class II, DR beta 1 precursor; MHC class II antigen DRB1*9; MHC class II HLA-DR beta 1 chain; SS1.

Gene ID

3123

Accession Number

NP_002116.2

Reactivity

Homo sapiens, Human

Target

HLA class II histocompatibility antigen, DRB1 beta chain, HLA class II histocompatibility antigen, DRB1-9 beta chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HLA-DRB1-9 ELISA Kit (Human) (OKEH03663) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HLA-DRB1-9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for HLA-DRB1-9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HLA-DRB1-9 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%

Sensitivity

0.322 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

30 kDa

Specificity

Natural and recombinant Human HLA class II histocompatibility antigen, DRB1-9 beta chain

Protein Length

266

NCBI Gene Symbol

HLA-DRB1

Protein Name

Q9TQE0

Gene Name URL

HLA-DRB1

Nucleotide Accession Number

NM_002125.3

Frequently Asked Questions

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