TRIL ELISA Kit (Rat)
Product Specifications
Gene Name
TLR4 interactor with leucine-rich repeats
Gene Aliases
Leucine-rich repeat-containing protein KIAA0644 homolog; RGD1310827; TLR4 interactor with leucine rich repeats.
Gene ID
362364
Accession Number
NP_001029182.1
Reactivity
Rat, Rattus norvegicus
Target
TLR4 interactor with leucine rich repeats
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TRIL ELISA Kit (Rat) (OKEH03645) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tril has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Tril is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tril captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
89 kDa
Specificity
Natural and recombinant Rat TLR4 interactor with leucine rich repeats
Protein Length
811
NCBI Gene Symbol
Tril
Protein Name
Q496Z2
Gene Name URL
Tril
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001034010.1
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