CEACAM1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
CEA cell adhesion molecule 1
Gene Aliases
ATP-dependent taurocolate-carrier protein; Bgp; BGPR; bone gamma-carboxyglutamic acid (Gla) protein; carcinoembryonic antigen related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) ; C-CAM 105; Ccam1; CD66a; CEA-related cell adhesion molecule 1 (bone gamma-carboxyglutamic acid (Gla) protein) (osteocalcin) ; cell-CAM 105; ecto-ATPase; GP110; osteocalcin; pp120; pp120/ecto-ATPase.
Gene ID
81613
Accession Number
NP_001029032.1
Reactivity
Rat, Rattus norvegicus
Target
Carcinoembryonic antigen-related cell adhesion molecule 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CEACAM1 ELISA Kit (Rat) (OKEH03643) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ceacam1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ceacam1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ceacam1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.164 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Rat Carcinoembryonic antigen-related cell adhesion molecule 1
Protein Length
519
NCBI Gene Symbol
Ceacam1
Protein Name
P16573
Gene Name URL
Ceacam1
Nucleotide Accession Number
NM_001033860.1
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