PPME1 ELISA Kit (Rat)
Product Specifications
Gene Name
Protein phosphatase methylesterase 1
Gene Aliases
PME-1; protein phosphatase methylesterase 1; RGD1309683.
Gene ID
361613
Accession Number
NP_001178767.1
Reactivity
Rat, Rattus norvegicus
Target
Protein phosphatase methylesterase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology PPME1 ELISA Kit (Rat) (OKEH03629) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Ppme1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ppme1 and incubated. The Ppme1 found in the sample or standards competes with the biotinylated Ppme1 for limited binding sites on the immobilized anti-human Ppme1 antibody. Excess unbound biotinylated Ppme1 and sample or standard Ppme1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ppme1 captured in the well and inversely proportional to the amount of Ppme1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Sensitivity
0.325 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Rat Protein phosphatase methylesterase 1
Protein Length
386
NCBI Gene Symbol
Ppme1
Protein Name
Q4FZT2
Gene Name URL
Ppme1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001191838.1
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