PRND ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Prion like protein doppel
Gene Aliases
AI450264; D; do; doppel; doppelganger; Dpl; prion protein dublet; prion protein-like protein; prion-like protein doppel; PrPLP.
Gene ID
26434
Accession Number
NP_001119810.1
Reactivity
Mouse, Mus musculus
Target
Prion-like protein doppel
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRND ELISA Kit (Mouse) (OKEH03628) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prnd has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Prnd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prnd captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
40 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
20 kDa
Specificity
Natural and recombinant Mouse Prion-like protein doppel
Protein Length
179
NCBI Gene Symbol
Prnd
Protein Name
Q9QUG3
Gene Name URL
Prnd
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001126338.2
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