FAP ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Fibroblast activation protein alpha
Gene Aliases
170 kDa melanoma membrane-bound gelatinase; dipeptidyl peptidase FAP; DPPIV; FAPA; FAPalpha; Fibroblast activation protein alpha; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; prolyl endopeptidase FAP; seprase; Serine integral membrane protease; SIMP; surface-expressed protease.
Gene ID
2191
Accession Number
NP_001278736.1
Reactivity
Homo sapiens, Human
Target
Prolyl endopeptidase FAP
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FAP ELISA Kit (Human) (OKEH03627) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for FAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
40 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
88 kDa
Specificity
Natural and recombinant Human Prolyl endopeptidase FAP
Protein Length
760
NCBI Gene Symbol
FAP
Protein Name
Q12884
Gene Name URL
FAP
Nucleotide Accession Number
NM_001291807.1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
GTR15211764 1 Vial
Lentiviral mouse Gdf10 shRNA (UAS) - Lentiviral mouse Gdf10 shRNA (UAS, RFP) (100)
Ask
View Details 
MBS1343845-01 0.02 mg (E-Coli)
Recombinant Yarrowia lipolytica Diphthamide biosynthesis protein 3 (DPH3)
Ask
View Details MBS1343845-02 0.1 mg (E-Coli)
Recombinant Yarrowia lipolytica Diphthamide biosynthesis protein 3 (DPH3)
Ask
View Details