CIRBP ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cold inducible RNA binding protein
Gene Aliases
A18 hnRNP; cold-inducible RNA-binding protein; glycine-rich RNA-binding protein CIRP.
Gene ID
81825
Accession Number
XP_006241076.1
Reactivity
Rat, Rattus norvegicus
Target
Cold-inducible RNA-binding protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CIRBP ELISA Kit (Rat) (OKEH03626) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cirbp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cirbp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cirbp captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Sensitivity
39.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Specificity
Natural and recombinant Rat Cold-inducible RNA-binding protein
Protein Length
172
NCBI Gene Symbol
Cirbp
Protein Name
P60825
Gene Name URL
Cirbp
Nucleotide Accession Number
XM_006241014.2
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