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CIRBP ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cold inducible RNA binding protein

Gene Aliases

A18 hnRNP; cold-inducible RNA-binding protein; glycine-rich RNA-binding protein CIRP.

Gene ID

81825

Accession Number

XP_006241076.1

Reactivity

Rat, Rattus norvegicus

Target

Cold-inducible RNA-binding protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CIRBP ELISA Kit (Rat) (OKEH03626) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cirbp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cirbp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cirbp captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/ml

Sensitivity

39.3 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

19 kDa

Specificity

Natural and recombinant Rat Cold-inducible RNA-binding protein

Protein Length

172

NCBI Gene Symbol

Cirbp

Protein Name

P60825

Gene Name URL

Cirbp

Nucleotide Accession Number

XM_006241014.2

Frequently Asked Questions

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