TREM2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
_x005F Mean Inter-assay CV%:
Gene Name
Triggering receptor expressed on myeloid cells 2
Gene Aliases
Trem; TREM-2; Trem2a; Trem2b; Trem2c; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c.
Gene ID
83433
Accession Number
NP_001259007.1
Reactivity
Mouse, Mus musculus
Target
Triggering receptor expressed on myeloid cells 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TREM2 ELISA Kit (Mouse) (OKEH03623) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Trem2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Trem2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Trem2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
41.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
25 kDa
Notes
Formerly GWB-C9ZU06
Specificity
Natural and recombinant Mouse Triggering receptor expressed on myeloid cells 2
Protein Length
227
NCBI Gene Symbol
Trem2
Protein Name
Q99NH8
Gene Name URL
Trem2
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001272078.1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
