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CD300LB ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

CD300 molecule like family member B

Gene Aliases

CD300 antigen like family member B; CD300 antigen-like family member B; Clm; CLM-; Clm7; CLM-7; CMRF35-like molecule 7; CMRF-35-like molecule-7; Gm253; immune receptor expressed on myeloid cells 3; IREM-3; leukocyte mono-Ig-like receptor 5; LM; LMIR5.

Gene ID

217304

Accession Number

NP_954691.2

Reactivity

Mouse, Mus musculus

Target

CMRF35-like molecule 7

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CD300LB ELISA Kit (Mouse) (OKEH03622) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd300lb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cd300lb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd300lb captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

41 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

24 kDa

Specificity

Natural and recombinant Mouse CMRF35-like molecule 7

Protein Length

209

NCBI Gene Symbol

Cd300lb

Protein Name

Q3U497

Gene Name URL

Cd300lb

Nucleotide Accession Number

NM_199221.2

Frequently Asked Questions

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