CD300LB ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
CD300 molecule like family member B
Gene Aliases
CD300 antigen like family member B; CD300 antigen-like family member B; Clm; CLM-; Clm7; CLM-7; CMRF35-like molecule 7; CMRF-35-like molecule-7; Gm253; immune receptor expressed on myeloid cells 3; IREM-3; leukocyte mono-Ig-like receptor 5; LM; LMIR5.
Gene ID
217304
Accession Number
NP_954691.2
Reactivity
Mouse, Mus musculus
Target
CMRF35-like molecule 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD300LB ELISA Kit (Mouse) (OKEH03622) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd300lb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cd300lb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd300lb captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
41 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
24 kDa
Specificity
Natural and recombinant Mouse CMRF35-like molecule 7
Protein Length
209
NCBI Gene Symbol
Cd300lb
Protein Name
Q3U497
Gene Name URL
Cd300lb
Nucleotide Accession Number
NM_199221.2
Frequently Asked Questions
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