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SLC7A11 ELISA Kit (Mouse)

Selected Variant

OKEH03605-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Solute carrier family 7 (cationic amino acid transporter, y+ system), member 11

Gene Aliases

9930009M05Rik; AI451155; amino acid transport system xc-; cysteine/glutamate transporter; cystine/glutamate transporter; sodium independent anionic amino acid transport system; solute carrier family 7 member 11; su; sut; xCT.

Gene ID

26570

Accession Number

NP_036120.1

Reactivity

Mouse, Mus musculus

Target

Cystine/glutamate transporter

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SLC7A11 ELISA Kit (Mouse) (OKEH03605) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc7a11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Slc7a11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc7a11 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

55 kDa

Specificity

Natural and recombinant Mouse Cystine/glutamate transporter

Protein Length

502

NCBI Gene Symbol

Slc7a11

Protein Name

Q9WTR6

Gene Name URL

Slc7a11

Nucleotide Accession Number

NM_011990.2

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