APC ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Adenomatosis polyposis coli
Gene Aliases
M, CC1, Min, mAPC, AI047805, AU020952, AW124434
Gene ID
11789
Accession Number
NP_031488.2
Reactivity
Mouse, Mus musculus
Target
Adenomatous polyposis coli protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology APC ELISA Kit (Mouse) (OKEH03604) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Apc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apc captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.166 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
311 kDa
Specificity
Natural and recombinant Mouse Adenomatous polyposis coli protein
Protein Length
2842
NCBI Gene Symbol
APC
Protein Name
Q61315
Gene Name URL
APC
Nucleotide Accession Number
NM_007462.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS6210684-01 0.1 mL
CD7 (T-cell Antigen CD7, GP40, T-cell Leukemia Antigen, T-cell Surface Antigen Leu-9, TP41) (MaxLight 550)
Sign In for Pricing
View Details MBS6210684-02 5x 0.1 mL
CD7 (T-cell Antigen CD7, GP40, T-cell Leukemia Antigen, T-cell Surface Antigen Leu-9, TP41) (MaxLight 550)
Sign In for Pricing
View Details 
P1649 100 µg
Recombinant human Breast cancer type 2 susceptibility protein
Sign In for Pricing
View Details