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RXRB ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Retinoid X receptor beta

Gene Aliases

AL023085; H-2R; H-2RIIBP; MHC class I regulatory element-binding protein H-2RIIBP; Nr2; Nr2b2; nuclear receptor subfamily 2 group B member 2; putative retinoid X receptor beta; RCo; RCoR-1; retinoic acid receptor RXR-beta; Retinoid X receptor beta; Ru; Rub.

Gene ID

20182

Accession Number

NP_001192145.1

Reactivity

Mouse, Mus musculus

Target

Retinoic acid receptor RXR-beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RXRB ELISA Kit (Mouse) (OKEH03597) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rxrb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Rxrb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rxrb captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%

Sensitivity

0.165 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

56 kDa

Specificity

Natural and recombinant Mouse Retinoic acid receptor RXR-beta

Protein Length

520

NCBI Gene Symbol

Rxrb

Protein Name

P28704

Gene Name URL

Rxrb

Nucleotide Accession Number

NM_001205216.1

Frequently Asked Questions

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