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SERPINA3N ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Serine (or cysteine) peptidase inhibitor, clade A, member 3N

Gene Aliases

Contrapsin-like protease inhibitor 6; CPi-26; serine protease inhibitor 2c; serine protease inhibitor 3; serine protease inhibitor A3N; serpin A3N; serpina3n-like protein; Spi-2.2; SPI3; SPI-3; Spin2c; Spin3.

Gene ID

24795

Accession Number

NP_113719.1

Reactivity

Rat, Rattus norvegicus

Target

Serine protease inhibitor A3N

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SERPINA3N ELISA Kit (Rat) (OKEH03593) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpina3n has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Serpina3n is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpina3n captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

47 kDa

Specificity

Natural and recombinant Rat Serine protease inhibitor A3N

Protein Length

418

NCBI Gene Symbol

Serpina3n

Protein Name

P09006

Gene Name URL

Serpina3n

Nucleotide Accession Number

NM_031531.1

Frequently Asked Questions

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