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PCOLCE ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Procollagen C-endopeptidase enhancer

Gene Aliases

PCPE; PCPE-1; procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer protein; procollagen COOH-terminal proteinase enhancer 1; procollagen C-proteinase enhancer 1; procollagen C-proteinase enhancer protein; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer.

Gene ID

29569

Accession Number

NP_062110.1

Reactivity

Rat, Rattus norvegicus

Target

Procollagen C-endopeptidase enhancer 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PCOLCE ELISA Kit (Rat) (OKEH03571) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pcolce has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pcolce is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pcolce captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/ml

Sensitivity

0.412 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

50 kDa

Specificity

Natural and recombinant Rat Procollagen C-endopeptidase enhancer 1

Protein Length

468

NCBI Gene Symbol

Pcolce

Protein Name

O08628

Gene Name URL

Pcolce

Nucleotide Accession Number

NM_019237.1

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