CYP2D4 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cytochrome P450, family 2, subfamily d, polypeptide 4
Gene Aliases
Cyp2d18; Cyp2d22; Cyp2d4v1; Cyp2d4v2; Cyp2d6; CYPIID18; CYPIID4; cytochrome P450 2d18; cytochrome P450 2D-29; cytochrome P450 2D-35; cytochrome P450 2D4; cytochrome P450, family 2, subfamily d, polypeptide 22; cytochrome P450, subfamily 2D, polypeptide 6; cytochrome P450, subfamily IID, polypeptide 6; Cytochrome P450, subfamily IID4; cytochrome P450-CMF3; cytochrome P450-DB4; debrisoquine 4-hydroxylase; P450 2D-29/2D-35; P450-CMF3; P450-DB4.
Gene ID
171522
Accession Number
NP_612524.1
Reactivity
Rat, Rattus norvegicus
Target
Cytochrome P450 2D4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CYP2D4 ELISA Kit (Rat) (OKEH03561) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp2d4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cyp2d4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp2d4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.182 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Rat Cytochrome P450 2D4
Protein Length
500
NCBI Gene Symbol
Cyp2d4
Protein Name
Q64680
Gene Name URL
Cyp2d4
Nucleotide Accession Number
NM_138515.2
Frequently Asked Questions
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