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CYP2D4 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cytochrome P450, family 2, subfamily d, polypeptide 4

Gene Aliases

Cyp2d18; Cyp2d22; Cyp2d4v1; Cyp2d4v2; Cyp2d6; CYPIID18; CYPIID4; cytochrome P450 2d18; cytochrome P450 2D-29; cytochrome P450 2D-35; cytochrome P450 2D4; cytochrome P450, family 2, subfamily d, polypeptide 22; cytochrome P450, subfamily 2D, polypeptide 6; cytochrome P450, subfamily IID, polypeptide 6; Cytochrome P450, subfamily IID4; cytochrome P450-CMF3; cytochrome P450-DB4; debrisoquine 4-hydroxylase; P450 2D-29/2D-35; P450-CMF3; P450-DB4.

Gene ID

171522

Accession Number

NP_612524.1

Reactivity

Rat, Rattus norvegicus

Target

Cytochrome P450 2D4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CYP2D4 ELISA Kit (Rat) (OKEH03561) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp2d4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cyp2d4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp2d4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.182 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

57 kDa

Specificity

Natural and recombinant Rat Cytochrome P450 2D4

Protein Length

500

NCBI Gene Symbol

Cyp2d4

Protein Name

Q64680

Gene Name URL

Cyp2d4

Nucleotide Accession Number

NM_138515.2

Frequently Asked Questions

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