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SH3YL1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

SH3 and SYLF domain containing 1

Gene Aliases

RAY; SH3 domain containing, Ysc84-like 1; SH3 domain-containing YSC84-like protein 1.

Gene ID

26751

Accession Number

NP_001153069.1

Reactivity

Homo sapiens, Human

Target

SH3 domain-containing YSC84-like protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SH3YL1 ELISA Kit (Human) (OKEH03548) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SH3YL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for SH3YL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SH3YL1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89.9%

Sensitivity

0.083 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

37 kDa

Specificity

Natural and recombinant Human SH3 domain-containing YSC84-like protein 1

Protein Length

342

NCBI Gene Symbol

SH3YL1

Protein Name

Q96HL8

Gene Name URL

SH3YL1

Nucleotide Accession Number

NM_001159597.2

Frequently Asked Questions

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