SH3YL1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
SH3 and SYLF domain containing 1
Gene Aliases
RAY; SH3 domain containing, Ysc84-like 1; SH3 domain-containing YSC84-like protein 1.
Gene ID
26751
Accession Number
NP_001153069.1
Reactivity
Homo sapiens, Human
Target
SH3 domain-containing YSC84-like protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SH3YL1 ELISA Kit (Human) (OKEH03548) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SH3YL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for SH3YL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SH3YL1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89.9%
Sensitivity
0.083 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Specificity
Natural and recombinant Human SH3 domain-containing YSC84-like protein 1
Protein Length
342
NCBI Gene Symbol
SH3YL1
Protein Name
Q96HL8
Gene Name URL
SH3YL1
Nucleotide Accession Number
NM_001159597.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
GMA-H5145-500ug 500 µg
Human GMF-beta Protein, His Tag (MALS verified)
Sign In for Pricing
View Details 
