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ATP6V1A ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

ATPase, H+ transporting, lysosomal V1 subunit A

Gene Aliases

70-kDa subunit; AI647066; Atp; Atp6a1; Atp6a2; Atp6v1a1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha 70 kDa; ATPase, H+ transporting, lysosomal 70kD, V1 subunit A; ATPase, H+ transporting, V1 subunit A; ATPase, H+ transporting, V1 subunit A1; lysosomal 70kDa; V; VA; VA68; vacuolar proton pump subunit alpha; V-ATPase 69 kDa subunit; V-ATPase subunit A; VPP2; V-type proton ATPase catalytic subunit A.

Gene ID

11964

Accession Number

NP_031534.2

Reactivity

Mouse, Mus musculus

Target

V-type proton ATPase catalytic subunit A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ATP6V1A ELISA Kit (Mouse) (OKEH03546) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp6v1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Atp6v1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp6v1a captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%

Sensitivity

0.17 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

68 kDa

Specificity

Natural and recombinant Mouse V-type proton ATPase catalytic subunit A

Protein Length

617

NCBI Gene Symbol

Atp6v1a

Protein Name

P50516

Gene Name URL

Atp6v1a

Nucleotide Accession Number

NM_007508.5

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