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EDAR ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Ectodysplasin A receptor

Gene Aliases

Anhidrotic ectodysplasin receptor 1; DL; downless homolog; downless, mouse, homolog of; ECTD10A; ECTD10B; ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; Ectodysplasin-A receptor; ED1R; ED3; ED5; EDA1R; EDA3; EDA-A1 receptor; EDA-A1R; HRM1; tumor necrosis factor receptor superfamily member EDAR.

Gene ID

10913

Accession Number

NP_071731.1

Reactivity

Homo sapiens, Human

Target

Tumor necrosis factor receptor superfamily member EDAR

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology EDAR ELISA Kit (Human) (OKEH03542) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human EDAR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated EDAR and incubated. The EDAR found in the sample or standards competes with the biotinylated EDAR for limited binding sites on the immobilized anti-human EDAR antibody. Excess unbound biotinylated EDAR and sample or standard EDAR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated EDAR captured in the well and inversely proportional to the amount of EDAR which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94.9%

Sensitivity

0.174 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

49 kDa

Specificity

Natural and recombinant Human Tumor necrosis factor receptor superfamily member EDAR

Protein Length

448

NCBI Gene Symbol

EDAR

Protein Name

Q9UNE0

Gene Name URL

EDAR

Nucleotide Accession Number

NM_022336.3

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