EDAR ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Ectodysplasin A receptor
Gene Aliases
Anhidrotic ectodysplasin receptor 1; DL; downless homolog; downless, mouse, homolog of; ECTD10A; ECTD10B; ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; Ectodysplasin-A receptor; ED1R; ED3; ED5; EDA1R; EDA3; EDA-A1 receptor; EDA-A1R; HRM1; tumor necrosis factor receptor superfamily member EDAR.
Gene ID
10913
Accession Number
NP_071731.1
Reactivity
Homo sapiens, Human
Target
Tumor necrosis factor receptor superfamily member EDAR
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology EDAR ELISA Kit (Human) (OKEH03542) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human EDAR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated EDAR and incubated. The EDAR found in the sample or standards competes with the biotinylated EDAR for limited binding sites on the immobilized anti-human EDAR antibody. Excess unbound biotinylated EDAR and sample or standard EDAR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated EDAR captured in the well and inversely proportional to the amount of EDAR which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94.9%
Sensitivity
0.174 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
49 kDa
Specificity
Natural and recombinant Human Tumor necrosis factor receptor superfamily member EDAR
Protein Length
448
NCBI Gene Symbol
EDAR
Protein Name
Q9UNE0
Gene Name URL
EDAR
Nucleotide Accession Number
NM_022336.3
Frequently Asked Questions
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