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PA1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

PAXIP1 associated glutamate rich protein 1

Gene Aliases

C16orf53; GAS; glutamate-rich coactivator associated with SRC1; glutamate-rich coactivator interacting with SRC1; glutamate-rich coactivator interacting with SRC1/NCOA1; PA1; PAXIP1-associated glutamate-rich protein 1; PAXIP1-associated protein 1; PTIP-associated 1 protein; PTIP-associated protein 1.

Gene ID

79447

Accession Number

NP_078792.1

Reactivity

Homo sapiens, Human

Target

PAXIP1-associated glutamate-rich protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology PA1 ELISA Kit (Human) (OKEH03538) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PA1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PA1 and incubated. The PA1 found in the sample or standards competes with the biotinylated PA1 for limited binding sites on the immobilized anti-human PA1 antibody. Excess unbound biotinylated PA1 and sample or standard PA1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PA1 captured in the well and inversely proportional to the amount of PA1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91.9%

Sensitivity

0.39 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

28 kDa

Specificity

Natural and recombinant Human PAXIP1-associated protein 1

Protein Length

254

NCBI Gene Symbol

PAGR1

Protein Name

Q9BTK6

Gene Name URL

PAGR1

Nucleotide Accession Number

NM_024516.3

Frequently Asked Questions

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