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TSTA3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

GDP-L-fucose synthase

Gene Aliases

3-5 epimerase/4-reductase; FX; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; GDP-L-fucose synthase; P35B; Protein FX; red cell NADP (H) -binding protein; SDR4E1; short chain dehydrogenase/reductase family 4E, member 1; Short-chain dehydrogenase/reductase family 4E member 1; testis tissue sperm-binding protein Li 45a; tissue specific transplantation antigen 3; tissue specific transplantation antigen P35B; TSTA3.

Gene ID

7264

Accession Number

NP_001304712.1

Reactivity

Homo sapiens, Human

Target

GDP-L-fucose synthase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology TSTA3 ELISA Kit (Human) (OKEH03537) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TSTA3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TSTA3 and incubated. The TSTA3 found in the sample or standards competes with the biotinylated TSTA3 for limited binding sites on the immobilized anti-human TSTA3 antibody. Excess unbound biotinylated TSTA3 and sample or standard TSTA3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TSTA3 captured in the well and inversely proportional to the amount of TSTA3 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94.9%

Sensitivity

0.172 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

36 kDa

Specificity

Natural and recombinant Human GDP-L-fucose synthase

Protein Length

321

NCBI Gene Symbol

GFUS

Protein Name

Q13630

Gene Name URL

GFUS

Nucleotide Accession Number

NM_001317783.1

Frequently Asked Questions

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