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POTEG ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

POTE ankyrin domain family member G

Gene Aliases

A26C2; ACTBL1; ANKRD26-like family C member 2; cancer/testis antigen family 104, member 4; CT104.4; POTE ankyrin domain family member G; POTE14; POTE-14; POTE14alpha; POTE22; prostate, ovary, testis-expressed protein on chromosome 14; protein expressed in prostate, ovary, testis, and placenta 14.

Gene ID

404785

Accession Number

NP_001005356.1

Reactivity

Homo sapiens, Human

Target

POTE ankyrin domain family member G

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology POTEG ELISA Kit (Human) (OKEH03536) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POTEG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for POTEG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POTEG captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%

Sensitivity

0.158 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

57 kDa

Specificity

Natural and recombinant Human POTE ankyrin domain family member G

Protein Length

508

NCBI Gene Symbol

POTEG

Protein Name

Q6S5H5

Gene Name URL

POTEG

Nucleotide Accession Number

NM_001005356.2

Frequently Asked Questions

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