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AGL ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Gene Aliases

Amylo-1, 6-glucosidase, 4-alpha-glucanotransferase; amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided; GDE; glycogen debrancher; glycogen debranching enzyme; glycogen debranching protein.

Gene ID

178

Accession Number

NP_000019.2

Reactivity

Homo sapiens, Human

Target

Glycogen debranching enzyme

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AGL ELISA Kit (Human) (OKEH03534) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for AGL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGL captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Sensitivity

0.086 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

175 kDa

Specificity

Natural and recombinant Human Glycogen debranching enzyme

Protein Length

1532

NCBI Gene Symbol

AGL

Protein Name

P35573

Gene Name URL

AGL

Nucleotide Accession Number

NM_000028.2

Frequently Asked Questions

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