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NFE2L2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Nuclear factor, erythroid derived 2, like 2

Gene Aliases

NFE2-related factor 2; NF-E2-related factor 2; Nr; Nrf2; nuclear factor erythroid 2-related factor 2; Nuclear factor, erythroid derived 2, like 2.

Gene ID

18024

Accession Number

NP_035032.1

Reactivity

Mouse, Mus musculus

Target

Nuclear factor erythroid 2-related factor 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NFE2L2 ELISA Kit (Mouse) (OKEH03530) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nfe2l2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Nfe2l2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nfe2l2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/ml

Sensitivity

40.1 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

67 kDa

Specificity

Natural and recombinant Mouse Nuclear factor erythroid 2-related factor 2

Protein Length

597

NCBI Gene Symbol

Nfe2l2

Protein Name

Q60795

Gene Name URL

Nfe2l2

Nucleotide Accession Number

NM_010902.4

Frequently Asked Questions

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