NFE2L2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Nuclear factor, erythroid derived 2, like 2
Gene Aliases
NFE2-related factor 2; NF-E2-related factor 2; Nr; Nrf2; nuclear factor erythroid 2-related factor 2; Nuclear factor, erythroid derived 2, like 2.
Gene ID
18024
Accession Number
NP_035032.1
Reactivity
Mouse, Mus musculus
Target
Nuclear factor erythroid 2-related factor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NFE2L2 ELISA Kit (Mouse) (OKEH03530) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nfe2l2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Nfe2l2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nfe2l2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Sensitivity
40.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 kDa
Specificity
Natural and recombinant Mouse Nuclear factor erythroid 2-related factor 2
Protein Length
597
NCBI Gene Symbol
Nfe2l2
Protein Name
Q60795
Gene Name URL
Nfe2l2
Nucleotide Accession Number
NM_010902.4
Frequently Asked Questions
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