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DST ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Dystonin

Gene Aliases

230 kDa bullous pemphigoid antigen;230/240 kDa bullous pemphigoid antigen; BP240; BPA; BPAG1; bullous pemphigoid antigen; bullous pemphigoid antigen 1; CATX15; CATX-15; D6S1101; DMH; DT; dystonia musculorum protein; dystonin; EBSB2; hemidesmosomal plaque protein; HSAN6; MACF2; trabeculin-beta.

Gene ID

667

Accession Number

NP_001138241.1

Reactivity

Homo sapiens, Human

Target

Dystonin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DST ELISA Kit (Human) (OKEH03522) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DST has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for DST is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DST captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91.9%

Sensitivity

0.081 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

861 kDa

Specificity

Natural and recombinant Human Dystonin

Protein Length

7570

NCBI Gene Symbol

DST

Protein Name

Q03001

Gene Name URL

DST

Nucleotide Accession Number

NM_001144769.2

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