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HTRA2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

HtrA serine peptidase 2

Gene Aliases

Epididymis secretory sperm binding protein; high temperature requirement protein A2; HtrA-like serine protease; MGCA8; OMI; Omi stress-regulated endoprotease; PARK13; protease, serine, 25; PRSS25; serine protease 25; serine protease HTRA2, mitochondrial; serine proteinase OMI.

Gene ID

27429

Accession Number

NP_037379.1

Reactivity

Homo sapiens, Human

Target

Serine protease HTRA2, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HTRA2 ELISA Kit (Human) (OKEH03514) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HTRA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for HTRA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HTRA2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91.9%

Sensitivity

0.079 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

49 kDa

Specificity

Natural and recombinant Human Serine protease HTRA2, mitochondrial

Protein Length

458

NCBI Gene Symbol

HTRA2

Protein Name

O43464

Gene Name URL

HTRA2

Nucleotide Accession Number

NM_013247.4

Frequently Asked Questions

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