CTCF ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
CCCTC-binding factor
Gene Aliases
11-zinc finger protein; AW108038; CCCTC-binding factor; CTCFL paralog; transcriptional repressor CTCF.
Gene ID
13018
Accession Number
NP_851839.1
Reactivity
Mouse, Mus musculus
Target
Transcriptional repressor CTCF
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Ctcf ELISA Kit (Mouse) (OKEH03508) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Ctcf antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ctcf and incubated. The Ctcf found in the sample or standards competes with the biotinylated Ctcf for limited binding sites on the immobilized anti-Mouse Ctcf antibody. Excess unbound biotinylated Ctcf and sample or standard Ctcf is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ctcf captured in the well and inversely proportional to the amount of Ctcf which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
39.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
84 kDa
Specificity
Natural and recombinant Mouse Transcriptional repressor CTCF
Protein Length
736
NCBI Gene Symbol
Ctcf
Protein Name
Q61164
Gene Name URL
Ctcf
Nucleotide Accession Number
NM_181322.3
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