What is the catalog number for PRPH ELISA Kit (Human)?

The catalog number for PRPH ELISA Kit (Human) is OKEH03505-96W.

What sizes are available for PRPH ELISA Kit (Human)?

PRPH ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is PRPH ELISA Kit (Human) in stock?

PRPH ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

PRPH ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Peripherin

Gene Aliases

NEF4; Neurofilament 4; neurofilament 4 (57kD) ; peripherin; PRPH1.

Gene ID

5630

Accession Number

NP_006253.2

Reactivity

Homo sapiens, Human

Target

Peripherin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology PRPH ELISA Kit (Human) (OKEH03505) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PRPH antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PRPH and incubated. The PRPH found in the sample or standards competes with the biotinylated PRPH for limited binding sites on the immobilized anti-human PRPH antibody. Excess unbound biotinylated PRPH and sample or standard PRPH is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PRPH captured in the well and inversely proportional to the amount of PRPH which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol