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TAOK1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

TAO kinase 1

Gene Aliases

HKFC-B; hTAOK1; KFC-B; kinase from chicken homolog B; MAP3K16; MARK Kinase; MARKK; microtubule affinity regulating kinase kinase; prostate-derived STE20-like kinase 2; prostate-derived sterile 20-like kinase 2; PSK2; PSK-2; serine/threonine protein kinase TAO1 homolog; serine/threonine-protein kinase TAO1; STE20-like kinase PSK2; TAO1; thousand and one amino acid protein 1; thousand and one amino acid protein kinase 1.

Gene ID

57551

Accession Number

NP_065842.1

Reactivity

Homo sapiens, Human

Target

Serine/threonine-protein kinase TAO1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TAOK1 ELISA Kit (Human) (OKEH03485) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TAOK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for TAOK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TAOK1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92.9%

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

116 kDa

Specificity

Natural and recombinant Human Serine/threonine-protein kinase TAO1

Protein Length

1001

NCBI Gene Symbol

TAOK1

Protein Name

Q7L7X3

Gene Name URL

TAOK1

Nucleotide Accession Number

NM_020791.2

Frequently Asked Questions

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