TAOK1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
TAO kinase 1
Gene Aliases
HKFC-B; hTAOK1; KFC-B; kinase from chicken homolog B; MAP3K16; MARK Kinase; MARKK; microtubule affinity regulating kinase kinase; prostate-derived STE20-like kinase 2; prostate-derived sterile 20-like kinase 2; PSK2; PSK-2; serine/threonine protein kinase TAO1 homolog; serine/threonine-protein kinase TAO1; STE20-like kinase PSK2; TAO1; thousand and one amino acid protein 1; thousand and one amino acid protein kinase 1.
Gene ID
57551
Accession Number
NP_065842.1
Reactivity
Homo sapiens, Human
Target
Serine/threonine-protein kinase TAO1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TAOK1 ELISA Kit (Human) (OKEH03485) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TAOK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for TAOK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TAOK1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92.9%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
116 kDa
Specificity
Natural and recombinant Human Serine/threonine-protein kinase TAO1
Protein Length
1001
NCBI Gene Symbol
TAOK1
Protein Name
Q7L7X3
Gene Name URL
TAOK1
Nucleotide Accession Number
NM_020791.2
Frequently Asked Questions
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