SOD3 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Superoxide dismutase 3
Gene Aliases
EC-SOD; ECSODPT; extracellular superoxide dismutase; extracellular superoxide dismutase [Cu-Zn]; Superoxide dimutase 3; superoxide dismutase 3, extracellular; superoxide dismutase B.
Gene ID
25352
Accession Number
NP_037012.1
Reactivity
Rat, Rattus norvegicus
Target
Extracellular superoxide dismutase [Cu-Zn]
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology SOD3 ELISA Kit (Rat) (OKEH03478) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sod3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Sod3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sod3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
<= 5.2%
Sensitivity
7.12 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
27 kDa
Specificity
Natural and recombinant Rat Extracellular superoxide dismutase [Cu-Zn]
Protein Length
244
NCBI Gene Symbol
Sod3
Protein Name
Q08420
Gene Name URL
Sod3
Nucleotide Accession Number
NM_012880.1
Frequently Asked Questions
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