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SOD3 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Superoxide dismutase 3

Gene Aliases

EC-SOD; ECSODPT; extracellular superoxide dismutase; extracellular superoxide dismutase [Cu-Zn]; Superoxide dimutase 3; superoxide dismutase 3, extracellular; superoxide dismutase B.

Gene ID

25352

Accession Number

NP_037012.1

Reactivity

Rat, Rattus norvegicus

Target

Extracellular superoxide dismutase [Cu-Zn]

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SOD3 ELISA Kit (Rat) (OKEH03478) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sod3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Sod3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sod3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

<= 5.2%

Sensitivity

7.12 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

27 kDa

Specificity

Natural and recombinant Rat Extracellular superoxide dismutase [Cu-Zn]

Protein Length

244

NCBI Gene Symbol

Sod3

Protein Name

Q08420

Gene Name URL

Sod3

Nucleotide Accession Number

NM_012880.1

Frequently Asked Questions

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