NR4A2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Nuclear receptor subfamily 4 group A member 2
Gene Aliases
HZF-3; immediate-early response protein NOT; intermediate-early receptor protein; NGFI-B/nur77 beta-type transcription factor homolog; NOT; nuclear receptor related 1; nuclear receptor subfamily 4 group A member 2; nur related protein-1, human homolog of; NURR1; orphan nuclear receptor NR4A2; orphan nuclear receptor NURR1; RNR1; T-cell nuclear receptor NOT; TINUR; transcriptionally inducible nuclear receptor related 1; transcriptionally-inducible nuclear receptor.
Gene ID
4929
Accession Number
NP_006177.1
Reactivity
Homo sapiens, Human
Target
Nuclear receptor subfamily 4 group A member 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NR4A2 ELISA Kit (Human) (OKEH03465) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NR4A2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for NR4A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NR4A2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
39.9 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 kDa
Specificity
Natural and recombinant Human Nuclear receptor subfamily 4 group A member 2
Protein Length
598
NCBI Gene Symbol
NR4A2
Protein Name
P43354
Gene Name URL
NR4A2
Nucleotide Accession Number
NM_006186.3
Frequently Asked Questions
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