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NR4A2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Nuclear receptor subfamily 4 group A member 2

Gene Aliases

HZF-3; immediate-early response protein NOT; intermediate-early receptor protein; NGFI-B/nur77 beta-type transcription factor homolog; NOT; nuclear receptor related 1; nuclear receptor subfamily 4 group A member 2; nur related protein-1, human homolog of; NURR1; orphan nuclear receptor NR4A2; orphan nuclear receptor NURR1; RNR1; T-cell nuclear receptor NOT; TINUR; transcriptionally inducible nuclear receptor related 1; transcriptionally-inducible nuclear receptor.

Gene ID

4929

Accession Number

NP_006177.1

Reactivity

Homo sapiens, Human

Target

Nuclear receptor subfamily 4 group A member 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NR4A2 ELISA Kit (Human) (OKEH03465) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NR4A2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for NR4A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NR4A2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

39.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

67 kDa

Specificity

Natural and recombinant Human Nuclear receptor subfamily 4 group A member 2

Protein Length

598

NCBI Gene Symbol

NR4A2

Protein Name

P43354

Gene Name URL

NR4A2

Nucleotide Accession Number

NM_006186.3

Frequently Asked Questions

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