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ANGPTL4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Angiopoietin-like 4

Gene Aliases

425O18-1; angiopoietin-like protein 4; angiopoietin-related protein 4; Arp4; Bk89; fasting-induced adipose factor; FI; Fiaf; fibrinogen/angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; HF; Hfarp; major histocompatibility complex region NG27; NG2; Ng27; Pgar; Pgarg; Pp1158; secreted protein Bk89.

Gene ID

57875

Accession Number

NP_065606.2

Reactivity

Mouse, Mus musculus

Target

Angiopoietin-related protein 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ANGPTL4 ELISA Kit (Mouse) (OKEH03450) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Angptl4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Angptl4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Angptl4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

0.059 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

46 kDa

Specificity

Natural and recombinant Mouse Angiopoietin-related protein 4

Protein Length

410

NCBI Gene Symbol

Angptl4

Protein Name

Q9Z1P8

Gene Name URL

Angptl4

Nucleotide Accession Number

NM_020581.2

Frequently Asked Questions

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