What is the catalog number for COQ10B ELISA Kit (Rat)?

The catalog number for COQ10B ELISA Kit (Rat) is OKEH03448-96W.

What sizes are available for COQ10B ELISA Kit (Rat)?

COQ10B ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is COQ10B ELISA Kit (Rat) in stock?

COQ10B ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

COQ10B ELISA Kit (Rat)

Selected Variant

OKEH03448-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Coenzyme Q10B

Gene Aliases

Coenzyme Q10 homolog B; coenzyme Q-binding protein COQ10 homolog B, mitochondrial; RGD1359509.

Gene ID

301416

Accession Number

NP_001009671.1

Reactivity

Rat, Rattus norvegicus

Target

Coenzyme Q-binding protein COQ10 homolog B, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology COQ10B ELISA Kit (Rat) (OKEH03448) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Coq10b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Coq10b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Coq10b captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Sensitivity

40.8 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

28 kDa

Specificity

Natural and recombinant Rat Coenzyme Q-binding protein COQ10 homolog B, mitochondrial

Protein Length

240

NCBI Gene Symbol

Coq10b

Protein Name

Q5I0I9

Gene Name URL

Coq10b

Nucleotide Accession Number

NM_001009671.1

Price

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