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SLC2A4 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Solute carrier family 2 member 4

Gene Aliases

Glucose transporter 4, insuline-responsive; glucose transporter type 4, insulin-responsive; Glut4; GLUT-4; insulin responsive glucose transporter; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2 , member 4; solute carrier family 2, facilitated glucose transporter member 4.

Gene ID

25139

Accession Number

NP_036883.1

Reactivity

Rat, Rattus norvegicus

Target

Solute carrier family 2, facilitated glucose transporter member 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SLC2A4 ELISA Kit (Rat) (OKEH03439) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc2a4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc2a4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc2a4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 91%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

55 kDa

Specificity

Natural and recombinant Rat Solute carrier family 2, facilitated glucose transporter member 4

Protein Length

509

NCBI Gene Symbol

Slc2a4

Protein Name

P19357

Gene Name URL

Slc2a4

Nucleotide Accession Number

NM_012751.1

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