What is the catalog number for PIM1 ELISA Kit (Human)?

The catalog number for PIM1 ELISA Kit (Human) is OKEH03434-96W.

What sizes are available for PIM1 ELISA Kit (Human)?

PIM1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is PIM1 ELISA Kit (Human) in stock?

PIM1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

PIM1 ELISA Kit (Human)

Selected Variant

OKEH03434-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Pim-1 proto-oncogene, serine/threonine kinase

Gene Aliases

Oncogene PIM1; PIM; pim-1 oncogene (proviral integration site 1) ; proto-oncogene serine/threonine-protein kinase pim-1; serine/threonine-protein kinase pim-1.

Gene ID

5292

Accession Number

NP_001230115.1

Reactivity

Homo sapiens, Human

Target

Serine/threonine-protein kinase pim-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PIM1 ELISA Kit (Human) (OKEH03434) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PIM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIM1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

15.77 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

45 kDa

Specificity

Natural and recombinant Human Serine/threonine-protein kinase pim-1

Protein Length

404

NCBI Gene Symbol

PIM1

Protein Name

P11309

Gene Name URL

PIM1

Nucleotide Accession Number

NM_001243186.1

Price

Frequently Asked Questions

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